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DeltaRex-G is a tumor-targeted MLV-based retroviralexpression vector (100 nm) displaying an amphotropic (panCol)SIG-targeted envelope on its surface to seek-out anaplastic proteinsexposed pathologically within the tumor microenvironment [40];the DeltaRex-G retrovector encodes a cytocidal Cyclin G1 inhibitor,dnG1 (Figure 3A), under the control of a hybrid CMV/LTR promoter[41]. The vector contains the neomycin resistance gene (neo) drivenby the SV40 early promoter; neo expression is useful for certifyingfunctional vector titers (gene transfer units/ml) and also fortracking / marking of transgene expression in vivo. However, in thecase of a tumor-targeted vaccine (DeltaVax), the neo marker genewas replaced with HSV-tk thymidine kinase gene, a so-called suicidetransgene for additional safety: (i) to avoid potential engraftment,(ii) to enable pharmacological control of gene expression withherpetic prodrugs, and (iii) to restrict GM-CSF expression locallywithin tumors [41].
Real-time in-situ synchrotron x-ray diffraction measurements were performed at the Cornell High Energy Synchrotron Source to characterize both the texture evolution and stresses within the individual texture components of Ag films during recrystallization. As deposited films had a nearly perfect (111) fiber texture. During isothermal anneals, stress and texture were characterized in real-time as the texture evolved into a strong (001) fiber. An Avrami analysis of the evolving texture fractions yielded very different activation energies for films on different barrier layers, suggesting different governing mechanisms were responsible for secondary grain growth. The strains were used to test a common model for texture prediction that assumes the same strain within each texture component. It was found that secondary (001) grains were able to grow primarily strain free. Selection for this strain energy minimizing orientation occurred during the nucleation process during which texture interactions play an important role. By using these real time measurements, we are able to show that driving forces for texture transformations in metal films may not be as simple previously described. 2ff7e9595c
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